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#9250 Ascaris IgG ELISA
ELISA kit for the diagnosis of human ascariasis
Parasitology and clinical relevance
Ascariasis
is caused by some soil transmitted helminths of the genus Ascaris. Humans can be infected by
ingesting infectious eggs upon contaminated soil, water or food. Most infected people do not show any symptoms. However, in
some cases, symptoms appear at the lung stage (persistent cough, shortness of
breath and wheezing) or at the intestinal stage (abdominal pain, nausea, vomiting,
and diarrhea). Diagnosis is based on the detection of eggs in stool, but,
in many cases it is not conclusive for the diagnosis. Serological assays
has an important place on the final diagnosis of the disease.
Test-Kit
96 determinations of IgG antibodies using standard methodologies.
Wells sensitized with Ascaris coelomic soluble antigens.
8-wells polystyrene breakable strips: useful and economic format for laboratories with a small workload.
CE registration: CH-202205-0023 |
Sensitivity and specificity
The test detects IgG antibodies
against parasites of Ascaris genus in human serum.
A sensitivity of 81%
was found with 27 sera positive with A. suum crude extract antigen and
negative with Toxocara canis Bordier ELISA kit.
A specificity of 96% was
found with 181 sera of blood donors (Swiss).
A specificity
of 98% was
found with 96 sera from patients of an infectiology unit (Swiss).
A specificity of
75% was
found with 44 sera positive with A. suum crude extract antigen and
positive with Toxocara canis Bordier ELISA kit.A specificity of
57%
was found with 65 sera of patients with other parasitic infections. |
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Contents
96 wells coated with Ascaris
coelomic soluble antigens.
Dilution
buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A - AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
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Ordering information
#9250 Ascaris IgG ELISA (96 assays)
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References
Schneider, R. and Auer, H.
(2016) Incidence
of Ascaris suum-specific antibodies in Austian
patients with suspected larva migrans visceralis (VLM) syndrome. Parasitology
research 115.
Dana,
D., Vlaminck, J., Tadege, B., Mekonnen, Z., Geldhof, P. et al. (2020)
Evaluation
of copromicroscopy and serology to measure the exposure to Ascaris
infections across age groups and to assess the impact of 3 years of biannual
mass drug administration in Jimma Town, Ethiopia.
Plos Negl
Trop Dis 14.
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European
directive 98/79/EC registration
Material Safety Data Sheet: Eng
- Fra - Deu
Bordier Affinity Products SA,
Chatanerie 2, Bātiment Biokema, CH-1023 Crissier, Switzerland
Phone: + 41 21 633 31 67. Fax: + 41 21
633 31 78. Email: cb(at)bordier.ch
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